Detecção de núcleoproteína-RNA do vírus rábico em diversos órgãos fora do sistema nervoso central de morcegos hematófagos infectados naturalmente

Vieira L. F. P., Pereira S. R. F. G., Galante A. C., Castilho J.G., Oliveira R.N., Brandao P. E. & Kotait I. 2011. Detection of rabies virus nucleoprotein-RNA in several organs outside the central nervous system in naturally-infected vampire bats. Pesquisa Veterinaria Brasileira 31(10):922-925. Setor de Virologia e Viroses, Laboratorio de Sanidade Animal, Hospital Veterinario, Centro de Ciencias e Tecnologias Agropecuarias, Universidade Estadual do Norte Fluminense, Av. Alberto Lamego 2000, Parque California, Campos dos Goytacazes, RJ 28013-602, Brazil. E-mail: luizuenf@yahoo.com.br Rabies is a neurological disease, but the rabies virus spread to several organs outside the central nervous system (CNS). The rabies virus antigen or RNA has been identified from the salivary glands, the lungs, the kidneys, the heart and the liver. This work aimed to identify the presence of the rabies virus in non-neuronal organs from naturally-infected vampire bats and to study the rabies virus in the salivary glands of healthy vampire bats. Out of the five bats that were positive for rabies in the CNS, by fluorescent antibody test (FAT), viral isolation in N2A cells and reverse transcription - polymerase chain reaction (RT-PCR), 100% (5/5) were positive for rabies in samples of the tongue and the heart, 80% (4/5) in the kidneys, 40% (2/5) in samples of the salivary glands and the lungs, and 20% (1/5) in the liver by RT-PCR test. All the nine bats that were negative for rabies in the CNS, by FAT, viral isolation and RT-PCR were negative for rabies in the salivary glands by RT-PCR test. Possible consequences for rabies epidemiology and pathogenesis are discussed in this work.