Optical characterization of healthy and neovascularized human ocular tissues

An in-depth characterization of optical properties of human retinal and retinal pigment epithelium (RPE)/choroidal tissues has been performed. The indices of refraction of these ocular tissues were determined by applying Brewster's law. The inverse adding doubling method, based on the diffusion approximation and radiative transport theory is applied to the measured values of the total diffuse transmission, total diffuse reflection, and collimated transmission to calculate the optical absorption, and scattering of the human retinal and retinal pigment epithelium/choroidal tissues. The scattering anisotropy coefficients were calculated using an independent method which relates scattering angles and intensities. The resulting values have been analyzed using appropriate statistical methods.