Electrochemiluminescence of peroxydisulfate using flower-like Ag@Au-paper electrode and Pd@Au-assisted multiple enzymatic labels

•A 3D origami electrochemiluminescence immunodevice was developed based on dual-signal amplification strategy.•The novel flower-like Ag@Au-PWE was fabricated via a seed-mediated growth approach as sensing platform.•GOD functionalized Pd@Au NPs were synthesized as signal amplification label.•This proposed strategy on the μ-ECLOI described here could be easily extended for the detection of other relative biomarkers in our future work.