AB0261 Matrix Metalloproteinase-3 as A Biomarker of Histological Synovitis in Rheumatoid Arthritis

Background Rheumatoid arthritis (RA) is an autoimmune disease characterized by chronic synovitis which leads to bone destruction. Matrix metalloproteinase (MMP) 3 can activate other MMPs, degenerate extracellular components and aggravate inflammation. Previous studies have reported correlation of serum MMP-3 and clinical disease activity in RA. However, little was known about the relationship between MMP-3 and histological synovitis. Objectives To explore the correlation of serum and synovial MMP-3 and histological synovitis in RA. Methods Sixty-two patients with active RA were recruited and their serum MMP-3 was detected by ELISA. Synovial tissues were obtained from RA patients as well as 12 OA and 8 orthopedic arthropathies (Orth.A) patients. Serial tissue sections were stained with H&E and immunohistochemically for MMP-3, CD3, CD20, CD38, CD68, CD15 and CD34. Krenn9s synovitis score was assessed semi-quantitatively and the density of positive-staining cells were quantitatively determined. Results (1) In RA synovium, MMP-3 expressed strongly in the endochylema of lining cells (both macrophage-like and fibroblast-like synoviocytes) with absence in the sublining area and the percentage of MMP-3+ lining cells (median 47%, IQR 39–52%) was significantly higher than that in OA (median 20%, IQR 17–24%, P 4, n=27) and low grade (≤4, n=35) synovitis. The percentage of MMP-3+ lining cells in patients with high grade synovitis was significantly higher than that in patients with low grade synovitis (median 51%, IQR 47–56% vs median 42%, IQR 36–49%, P Conclusions Our results implied that MMP-3 is involved in synovitis and serum MMP-3 may be a helpful biomarker of histological synovitis. Acknowledgements This work was supported by Chinese National Natural Science Research Grant (grant no. 81373183 and 81001334), Specialized Research Fund for the Doctoral Program of Higher Education (grant no.20130171110075) and Guangdong Natural Science Foundation (grant no.S2013010014396). Disclosure of Interest None declared DOI 10.1136/annrheumdis-2014-eular.3622