Combined effect of ultrasound/SonoVue microbubble on CD4(+)CD25(+) regulatory T cells viability and optimized parameters for its transfection.

Chunying Shi,Yu Zhang,Haichao Yang,Tianxiu Dong,Yaodong Chen,Yutong Xu,Xiuhua Yang

Ultrasonics（2015）

引用 9|浏览6

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摘要

•The optimal ultrasound parameters were: MI=1.4, 150 or 180s exposure time.•The optimal microbubble parameter was 10% microbubble concentration.•Combined use ultrasound and microbubble can increase the transfection efficiency.

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关键词

Ultrasound,Microbubble,CD4+CD25+ regulatory T cells,Gene therapy,Hepatocellular carcinoma

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