Stable lentiviral transformation of CHO cells for the expression of the hemagglutinin H5 of avian influenza virus in suspension culture

•We obtained clones that stably expressed hemagglutinin in CHO cells by lentiviral vector transduction.•The growth kinetics and the stable production of the hemagglutinin protein were demonstrated in the clone with the highest expression level.•Hemagglutinin purification was carried out by immunoaffinity chromatography with a high degree of purity.•ELISA assays using the hemagglutinin protein direct from the supernatant or in its purified form showed similar antibody titers.