A simple method for desalting biological fluids for chromatography.

A simplified procedure for desalting biological fluids, the amino acid composition of which is to be determined by chromatographic technique,has been developed. Recovery experiments carried out have given results which are within the usual experimental error. The chromatograms from desalted samples are by far better than the ones obtained without desalting. The method has been compared with the electrolytic desalting procedure.