Non-invasive dry mass determination and monitoring at the single cell level with digital holographic microscopy
BIOPHOTONICS: PHOTONIC SOLUTIONS FOR BETTER HEALTH CARE(2008)
摘要
Digital holography microscopy (DHM) is an optical microscopy technique which allows recording non-invasively the phase shift induced by living cells with nanometric sensitivity. Here, we exploit the phase signal as an indicator of dry mass (related to the protein concentration). This parameter allows monitoring the protein production rate and its evolution during the cell cycle.
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关键词
digital holography,quantitative phase microscopy,protein production,dry mass,cell cycle,cell growth,fission yeast,Schizosaccharomyces pombe
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