In vitro mass propagation of Murraya koenigii L

•The study for the first time standardized an effective and reproducible in vitro regeneration protocol in Murraya koenigii.•Shoot tip explants were found highly effective for the large scale in vitro shootlets production.•An efficient root regeneration protocol has standardized in the shootlets by using in vitro liquid culture technique.•Developed shootlets were successfully acclimatized by utilizing 70 days acclimatization period.•The protocol could fruitfully contribute in the in vitro production and conservation of Murraya koenigii.