Enzyme-Free Glucose Sensor Based On Micro-Nano Dualporous Gold-Modified Screen-Printed Carbon Electrode

In this work, an enzyme-free glucose sensor at neutral pH (7.4) has been developed based on a micro-nano dualporous (MNDP) gold-modified screen-printed carbon electrode (SPCE). The combination of MNDP gold and SPCE makes the device a compact, low-cost and reliable enzyme-free glucose sensor. MNDP gold was directly synthesized via an electrochemical deposition method on a carbon surface using a hydrogen bubble as a dynamic template. The obtained MNDP gold has a highly porous structure and is mechanically stable. The MNDP gold-modified SPCE was applied to glucose sensing. This enzyme-free sensor showed a wide linear range, with the logarithm of glucose concentration between 1.5 and 16 mM, a high sensitivity of 48.4 mu A.mM(-1).cm(-2), and a limit of detection of 25 mu M. It also exhibited exquisite selectivity with the common interference compounds in real samples. Moreover, this sensor successfully detects glucose levels in human blood serum with satisfactory results and functional recovery.