In vitro shoot regeneration and flowering of sesame (Sesamum indicum L.) cv. SVPR-1.

In vitro shoot regeneration and flowering were achieved from shoot tip and nodal explants of Sesamum indicum L. on MS basal medium containing different combinations and concentrations of cytokinins and auxins. The highest percentage (91.8) of shoot regeneration and number of shoots (25.9) were observed from shoot tip explants cultured on MS basal medium supplemented with 2.0 mg l BAP and 0.3 mg l NAA combination. Flower buds (8.12) were obtained in shoot tip explants cultures. In vitro flowers and root induction were achieved in MS medium containing 1.5 mg l NAA and 0.03 mg l BAP. Subsequently, the plants were transferred to red soil: sand: cowdung (2:1:1 -v/v/v). The survival rate was 78 % in the greenhouse conditions.