Extraction of a Polysaccharide from the Skin of Ray Raja montagui by Free-Radical Depolymerization with Metallic Catalysis

The large production of sulfated polysaccharides was routinely investigated by various procedures such as enzymatic method and acid hydrolysis. The free-radical depolymerization applied directly on the skin of ray constitutes an efficient method and an original process to produce bioactive compounds in large amount and good reproducibility. The depolymerized sulfated polysaccharides fractions obtained from the skin of ray Raja montagui have an average molecular weight ranging from 29 kDa to 5 kDa and were endowed with higher anticoagulant activity compared to mammalian dermatan sulfate from intestinal mucosa. Moreover, the anticoagulant effect of these fractions was 4 and 5-fold higher than the DS from porcine mucosa as shown by activated partial thromboplastin time and thrombin time, respectively. In particular, this was illustrated by lower IC50 as for the overall inhibition of thrombin in human plasma.