Experimental Methods For Registration Of Electrical Activity Of The Heart On Cell And Whole Organ Level

Modern touchiess recording systems for registration of electrical activity of the heart are based on application of voltage sensitive dye (VSD) into the examined tissue. Excited VSD's undergo changes in their electronic structure, and consequently their fluorescence spectra, in response to changes in the surrounding electric field. Fast VSDs are consistently sensitive probes and can be used in electrophysiological studies in beating hearts. Spectral properties of VSD's determine the use of common lamps for excitation of dyes and photodiode detectors or fast digital cameras to register changes in emitted fluorescence light.