Genetic Response of Different Genotypes of Sugarcane at Different Tissue Culture Techniques Combinations

Sugarcane is one of the major sugar crops of the world. Tissue culture study is mainly aimed to produce disease free plants. The study was carried out to find out the best combinations of tissue culture technique used for sugarcane to produce it asexually. It was also aimed to find out the best responsive genotype for tissue culture. From the five genotypes taken for the tissue culture study (HSF-242, CPF-246, CPF-247, S2002US-618, S2002US-718), Callogenesis study revealed significant differences for different genotypes and treatments. The genotype HSf-242 showed maximum callus formation at 3mg/l of 2, 4-D level but not best performance for organogenesis. For callus least performing genotype was S2002US-718. For organogenesis CPF-247 performed well at all treatments.