Cd20(+) T Cells Have A Predominantly Tc1 Effector Memory Phenotype And Are Expanded In The Ascites Of Patients With Ovarian Cancer
ONCOIMMUNOLOGY(2015)
摘要
Recently, a small subset of T cells that expresses the B cell marker CD20 has been identified in healthy volunteers and in patients with rheumatoid arthritis and multiple sclerosis. The origin of these CD20-positive T cells as well as their relevance in human disease remains unclear. Here, we identified that after functional B cell/T cell interaction CD20 molecules are transferred to the cell surface of T cells by trogocytosis together with the established trogocytosis marker HLA-DR. Further, the presence of CD20 on isolated CD20(+) T cells remained stable for up to 48h of ex vivo culture. These CD20(+) T cells almost exclusively produced IFN gamma (similar to 70% vs. similar to 20% in the CD20(+) T cell population) and were predominantly (CD8(+)) effector memory T cells (similar to 60-70%). This IFN gamma producing and effector memory phenotype was also determined for CD20(+) T cells as detected in the peripheral blood and ascitic fluids of ovarian cancer (OC) patients. In the latter, the percentage of CD20(+) T cells was further strongly increased (from similar to 6% in peripheral blood to 23% in ascitic fluid). Taken together, the data presented here indicate that CD20 is transferred to T cells upon intimate T cell/B cell interaction. Further, CD20(+) T cells are of memory and IFN gamma producing phenotype and are present in increased amounts in ascitic fluid of OC patients.
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关键词
Ascites,cancer immunology,CD20,ovarian cancer,trogocytosis
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