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Efficient Multiplexed Integration of Synergistic Alleles and Metabolic Pathways in Yeasts Via CRISPR-Cas.

Andrew A. Horwitz,Jessica M. Walter,Max G. Schubert,Stephanie H. Kung,Kristy Hawkins,Darren M. Platt,Aaron D. Hernday,Tina Mahatdejkul-Meadows,Wayne Szeto,Sunil S. Chandran,Jack D. Newman

Methods in Molecular Biology Yeast Systems Biology（2019）

引用 301|浏览26
关键词
CRISPR,Cas9,gRNA,Yeast,Multiplex,Allele,Metabolic engineering,Synthetic biology
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