Femtosecond near-infrared opto-injection of single living cells: pore size in dependence of laser intensity

We investigate the size of transient artificial pores as a function of the incident laser intensity in femtosecond near-infrared laser opto-injection into single living Bovine aortic endothelial cells (BAECs). Molecules ranging in size from 457 dalton to 500kD were used in size exclusion experiments. We found that the threshold laser intensity for pore creation was in dependence of the size of the molecule.