Establishment Of A Rat Model Of Radiation-Induced Lung Injury

Background: Radiation-induced lung injury is a refractory side effect in lung cancer radiotherapy, the mechanism still remains unclear, hence an appropriate animal mode may become useful to investigate it. Materials and Methods: 50 female Wistar rats were randomly divided into 5 groups, average 10 rats/cage: A. control group; B. 3Gyx10f; C. 6Gyx5f; D. 12.5Gyx1f; E.15.3Gyx1f. By different doses of radiation to the right lung of rats under American Varian linear accelerator, all the rats were executed at 30days after irradiation, lung tissues were collected to observe the pathological changes by HE staining, and additional lung tissues were extracted nnRNA to measure gene expression of TNF-a and IL-18 by qRT-PCR. Results: In contrast to the control group, congestion, gore, edema, broadening alveolar interval and the bulla can be observed after irradiation. 3Gyx1Of radiation led to most rat death because of frequent anaesthesia. 12.5Gyx1f radiation induced mild lung injury. 6Gyx5f and 15.3Gyx1f radiation induced remarkable lung injury. However, 15.3Gyx1f was seldom adopted in clinical settings. The measurement of cytokine variation by qRT-PCR also indicated that 6Gyx5f radiation stimulated the most obvious elevation of IL-18, while 15.3Gyx1f radiation elevated TNF-a most. Conclusion: A rat model of radiation-induced lung injury was appropriately established, 6Gyx5f radiation was the most suitable radiation method for establishing this rat model.