Agrobacterium-Mediated Transformation Of Carotenoid Synthesis Gene In Kiwifruit (Actinidia Deliciosa 'Hayward')

To improve the quality of kiwifruit (Actinidia deliciosa 'Hayward'), the research team transformed with Agrobacterium tumefaciens EHA105 harboring the binary vector pCAMBIA 1300 associated with carotenoid synthesis gene such as geranylgeranyl diphosphate synthase (GGPS), phytoene desaturase (PDS), and zeta-carotene desaturase (ZDS), respectively. Also, the binary vector had a hygromycin resistance gene (hptII) as a selectable marker. Plant materials were prepared by micro-cross sectioning system of stems (sectioning of 8011 Pin). The transformed plant was selected on 1/2 MS medium containing 4.5x.10(-3) mu M of 2,4-dichlorophenoxyacetic acid (2,4-D) and 4.6x10(-1) mu M of zeatin, 5 mu g ml(-1) hygormycin, and 500 mu g ml(-1) cefotaxime. Shoot regeneration efficiency of treated explants were 13.3% GGPS, 4.3% PDS, and 16.4% ZDS. Also, the confirmation of transformants was analyzed by PCR. The transformation efficiencies were 8% GGPS, 2.9% PDS, and 12.3% ZDS, respectively.