Association Of Tumor Necrosis Factor Alpha Gene Polymorphisms With Inflammatory Bowel Disease In Iran

Background: Inflammatory bowel disease (IBD) is a chronic disease of unknown etiology, in which genetic factors, seem to play an important role in the disease predisposition and course. Assessment of tumor necrosis factor (TNF-alpha) gene polymorphisms in many populations showed a possible association with IBD. Considering the genetic variety in different ethnic groups, the aim of the present study was to investigate the association of five important single nucleotide polymorphisms (SNPs) in the promoter region of (TNF-alpha) gene with IBD in Iran.Methods: In this case-control study, 156 Ulcerative colitis (UC) patients, 50 Crohn's disease (CD) patients and 200 sex and age matched healthy controls of Iranian origin were enrolled. The study was performed during a two year period (2008-2010) at Taleghani Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran. DNA samples were evaluated for (TNF-alpha) gene polymorphisms (including -1031, -863, -857, -308 and -238) by PCR and RFLP methods.Results: The frequency of the mutant allele of -1031 polymorphism was significantly higher in Iranian patients with Crohn's disease compared to healthy controls (P=0.01, OR=1.92; 95% CI: 1.14-3.23). None of the other evaluated polymorphisms demonstrated a significant higher frequency of mutant alleles in Iranian IBD patients compared to controls.Conclusion: Among the five assessed (SNPs), only -1031 polymorphism of (TNF-alpha) gene may play a role in disease susceptibility for Crohn's disease in Iran. This pattern of distribution of (TNF-alpha) gene polymorphisms could be specific in this population.