Determining lower limits of detection of digital PCR assays for cancer-related gene mutations

Coren A Milbury,Qun Zhong, Jesse Lin, Miguel Williams,Jeff Olson,Darren R Link,Brian J Hutchison

Biomolecular Detection and Quantification（2014）

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摘要

•Digital PCR offers very high assay sensitivity and limit of detection.•An approach for calculating limit of detection is demonstrated for two EGFR assays.•Assay LoDs have been evaluated for eighteen cancer targets.

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关键词

PCR,EGFR,LoB,LoD,N,NWT,NMut,λ,p,R,ΛFP,RFP

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