e0110 The effects of atorvastatin on C-reactive protein induced Toll-like receptor 4 expression on CD14+ monocyte

Objective To observe the effects of atorvastatin on C-reactive protein (CRP) induced Toll-like receptor 4 expression on CD14 + monocyte in human, and anti-inflammatory effect of atorvastatin. Methods CD14 + monocytes were isolated from blood in healthy volunteers by the Ficoll density gradient and stimulated by CRP with different concentrations (5, 25, 50, 100 μg/ml) and different exposure time (50 μg/ml CRP coincubated for 6, 12, 24 and 48 h). The protein expression of TLR4 was measured by flow cytometry and mRNA expression of TLR4 and MD-2 were tested by quantitative PCR. Measurements of TNFα, IL-6 and MMP-9 in the supernatants of cultured monocytes were performed by ELISA. Results CRP (5, 25, 50 and 100 μg/ml) increased dose-dependently the expression of TLR4 protein (32.22±2.80%, 49.94±5.58%, 74.82±3.24% and 90.82±2.88%; p + monocytes for various times (6, 12, 24 and 48 h) and also increased time-dependently the expression of TLR4 protein (29.80±2.70%, 47.44±4.41%, 81.71±2.92% and 50.57±3.34%; p Conclusion CRP can increase dose-dependently and time-dependently the expression of TLR4 on CD14 + monocyte in human, and the production of TNFα, IL-6 and MMP-9 in CD14 + monocyte. Atorvastatin can inhibit dose-dependently the expression of TLR4 mRNA and protein induced by CRP and the release of TNFα, IL-6 and MMP-9 in CD14 + monocytes in human. Atorvastatin has anti-inflammatory effects and may restrain innate immune response in vitro by inhibition of monocyte Toll-like receptor signalling.