GW24-e3111 Comparations of two common mouse models of chronic heart failure by echocardiography

Objectives To compare the recorded echocardiographic parameters in two common mouse models of chronic heart failure. Methods KM mice were subjected to transverse aortic constriction (TAC), myocardial infarction induced by coronary artery ligation (CAL), or sham operation. Mice were characterised by echocardiography 4- to 8-week post-surgery. Results Early operative mortality (within 24 h) was not significantly different between CAL (5/14) and TAC (3/10). No mortality was achieved during 7 days after the surgical procedure. The mice died from LV rupture in MI cases, defined by excessive blood filling the chest cavity. No mice died between 2 and 4 weeks after surgery. All mice had severe left ventricular hypertrophy, impaired systolic function and pulmonary congestion compared with sham controls. 4-week after surgery TAC-operated and sham-operated mice showed significant changes in diastolic interventricular septum thickness (IVSd); CAL-operated and sham-operated mice showed significant changes in systolic and diastolic left ventricular internal diameter (LVIDd and LVIDs). 4-week’s ejection fraction (EF) of the CAL-operation group is (47.71 ± 8.07)% compared with (58.33 ± 6.31)% of the TAC-operation group and (76.53 ± 5.96)% of the sham operation group; fraction shortening (FS) of the CAL-operation group is (24.26 ± 4.34)% compared with (30.27 ± 4.08)% of the TAC-operation group and (44.54 ± 5.14)% of the sham operation group. While 8-week’s ejection fraction (EF) of the TAC-operation group is (36.12 ± 13.35)%; fraction shortening (FS) of the TAC-operation group is (17.53 ± 6.69)%. Conclusions CAL-operation group shared a higher mortality because of the direct damage to the myocardium, which leads to postoperative arrhythmias and other adverse events. Cardiac function decline sharply in MI group, while TAC postoperative cardiac decompensation performance after 8 weeks. Characteristics of these two models in morphology: CAL-operated mice occupy the thinner wall and enlarged ventricular cavity; while septal hypertrophy and ventricular chamber volume changes can be observed in TAC-operated mice.