Quasi-continuous fermentation in a reverse-flow diafiltration bioreactor

Kristina Meier,Frederike Carstensen,Matthias Wessling,Lars Regestein,Jochen Buchs

Biochemical Engineering Journal（2014）

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摘要

•RFD is a membrane based cell retention system alternating feeding and harvesting.•Alternations result in feed pulses causing short-term oxygen limitations.•Short-term oxygen limitations of Hansenula polymorpha decrease product yield by 25%.•Limitations are enforced at increasing dilution rates.•Sequential operation of membranes result in continuous feeding without limitations.

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关键词

In situ product recovery,Hansenula polymorpha,Hollow fibers,Membrane bioreactors,Dissolved Oxygen,Glucose pulse

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