Distinct Roles Of The Mucus Layer And Microbiota In Conferring Innate Host Defense And Susceptibility To Disease

The human intestine has approximately 100 trillion microorganisms, mainly bacteria that form the microbiota. These microorganisms play a key role in digestive health as well as in the development of a balanced immune system. The intestinal mucosa is covered by MUC2 mucus that acts as a protective barrier. Alteration in microbiota composition (dysbiosis) and the mucus barrier is common in several gastrointestinal pathologies. The distinct contribution of the microbiota and the mucus barrier play in the pathogenesis of colitis is not well understood. The aim of this study was to determine whether shifts in microbiota could alter the onset and progression of colitis in Muc2 mucin deficient and sufficient mice. F2 littermates Wt (Muc2+/+) and homozygous (Muc2-/-) mice were gavaged with antibiotics, fecal transplanted with their littermate stool and susceptibility to dextran sulphate sodium (DSS) was quantified. Fecal samples pre- and post-antibiotic was analyzed by MiSeq lll lluminar sequencing. Intestinal pe...