Display of Thermotoga maritima MSB8 nitrilase on the spore surface of Bacillus subtilis using out coat protein CotG as the fusion partner

•An effective prokaryotic immobilization method based on surface display system was established.•The Thermotoga maritima MSB8 nitrilase was successfully displayed on the spore surface of B. subtilis DB 403 verified by western blot analysis and activity measurement.•The thermal stability, pH stability of Thermotoga maritima MSB8 nitrilase were both improved after displayed on the spore surface of B. subtilis DB 403.•The activity of spore surface-displayed nitrilase was not significantly decreased throughout the reusability process, which still retained 83% of the initial activity at the fifth cycle.