Large α-aminonitrilase activity screening of nitrilase superfamily members: Access to conversion and enantiospecificity by LC–MS

Franck Bordier,Mark Stam,Ekaterina Darii,Sabine Tricot, Aurélie Fossey, Johanna Rohault,Adrien Debard,Aline Mariage,Virginie Pellouin,Jean-Louis Petit,Alain Perret,David Vallenet,Marcel Salanoubat,Jean Weissenbach,Carine Vergne-Vaxelaire,Véronique de Berardinis,Anne Zaparucha

Journal of Molecular Catalysis B: Enzymatic（2014）

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摘要

•We performed a large nitrilase activity screening on α-aminonitriles.•The enzyme collection covers the whole biodiversity of the nitrilase superfamily.•An efficient LC-MS/MS method gave access to both conversion and enantiospecificity.•Five new α-aminonitrilases were found, with no nitrile hydratase activity.•(S)-Norvaline, (R)-phenylglycine and two phenylglycine derivatives were obtained.

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关键词

Alpha-aminonitrile,Nitrilase,LC–MS screening,Enantiospecificity

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