Nicotine Administration Attenuates Methamphetamine-Induced Novel Object Recognition Deficits

Background: Previous studies have demonstrated that methamphetamine abuse leads to memory deficits and these are associated with relapse. Furthermore, extensive evidence indicates that nicotine prevents and/or improves memory deficits in different models of cognitive dysfunction and these nicotinic effects might be mediated by hippocampal or cortical nicotinic acetylcholine receptors. The present study investigated whether nicotine attenuates methamphetamine-induced novel object recognition deficits in rats and explored potential underlying mechanisms.Methods: Adolescent or adult male Sprague-Dawley rats received either nicotine water (10-75 mu g/mL) or tap water for several weeks. Methamphetamine (4 x 7.5 mg/kg/injection) or saline was administered either before or after chronic nicotine exposure. Novel object recognition was evaluated 6 days after methamphetamine or saline. Serotonin transporter function and density and alpha 4 beta 2 nicotinic acetylcholine receptor density were assessed on the following day.Results: Chronic nicotine intake via drinking water beginning during either adolescence or adulthood attenuated the novel object recognition deficits caused by a high-dose methamphetamine administration. Similarly, nicotine attenuated methamphetamine-induced deficits in novel object recognition when administered after methamphetamine treatment. However, nicotine did not attenuate the serotonergic deficits caused by methamphetamine in adults. Conversely, nicotine attenuated methamphetamine-induced deficits in alpha 4 beta 2 nicotinic acetylcholine receptor density in the hippocampal CA1 region. Furthermore, nicotine increased alpha 4 beta 2 nicotinic acetylcholine receptor density in the hippocampal CA3, dentate gyrus and perirhinal cortex in both saline-and methamphetamine-treated rats.Conclusions: Overall, these findings suggest that nicotine-induced increases in alpha 4 beta 2 nicotinic acetylcholine receptors in the hippocampus and perirhinal cortex might be one mechanism by which novel object recognition deficits are attenuated by nicotine in methamphetamine-treated rats.