Evaluation of Root Canal Debridement of Human Molars Using the GentleWave System

•Recently, a novel device, the GentleWave System was shown to have a higher tissue dissolution rate in a simulated tooth model than traditional and contemporary endodontic methods.•We evaluated the tissue debris removed from the root canal system after it was cleaned using the GentleWave System and compared it with those cleaned using conventional instrumentation with needle irrigation.•The GentleWave System cleaned approximately 97.2% of tissue debris in the apical and middle region of mesial roots of mandibular molars.•An increase in the cleaning quality of complex anatomic regions such as the isthmus regions was observed when teeth were cleaned with the GentleWave System as compared to traditional methods.