Developing bioelectrical impedance analysis methods for age‐0 brook trout

Year class strength of many fishes often is determined by survival through the first winter. Increased fat reserves improve survival and overall cohort success. Bioelectrical impedance analysis (BIA) methods are established for estimating proximate composition of adult brook trout, Salvelinus fontinalis (Mitchill), but none have been developed for early life stages. Small-fish BIA would provide information about age-0 percent dry weight, a proxy for fat reserves, allowing for better prediction of cohort success. The objective of this study was to develop BIA methods that provide reliable estimates of percent dry weight for age-0 brook trout. BIA measurements were taken at seven anatomical locations from 48 to 115mm fish. A model developed using BIA measures taken by subdermal needle electrodes precisely predicted percent dry weight (best model, RMSE=1.03, R-2=0.86). Although lacking support, as determined by information theoretical analysis, BIA measured with non-invasive external rod electrodes also precisely predicted percent dry weight (RMSE=1.09, R-2=0.85). Models developed using two electrode locations performed better than models developed with only one location. For small brook trout, a dorsal to ventral pre-dorsal fin electrode location should be used in conjunction with a dorsal total length location when measuring BIA to predict percent dry weight.