Induction of wheat/barley translocations by irradiation and their detection using fluorescence in situ hybridization
Acta Agronomica Hungarica(2010)
摘要
The aim of the present study was to test the efficiency of gamma irradiation in inducing translocations between wheat and barley genomes using addition lines. The Martonvásári 9 kr1-Igri disomic addition set, previously produced in Martonvásár, was irradiated with gamma rays. The pattern of irradiation-induced intergenomic chromosome rearrangements was analysed in the mutagenized (M0) generation by genomicin situhybridization (GISH). Centric fusions and a wide variety of reciprocal, terminal and interstitial translocations were frequently induced. The intergeneric translocations produced here are expected to be stabilized in later backcross progenies as a set of introgression lines carrying few but distinct rearrangements.
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关键词
in situ hybridization,gamma irradiation,translocations
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