Solid-phase DNA isolation from food matrices using hydrophilic magnetic microspheres

Štěpánka Trachtová,Alena Španová,Judit Tóth,Zsolt Prettl,Daniel Horák,János Gyenis,Bohuslav Rittich

Food and Bioproducts Processing（2015）

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摘要

•Determination of the effect of PEG 600 and PEG 6000 on DNA coil diameter.•Development of hydrophilic non-porous magnetic microspheres containing carboxyl groups.•Correlation of the amount of isolated DNA with the content of COOH groups on the microspheres.•Isolation of PCR-ready DNA from food samples by magnetic microspheres.

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关键词

DNA compaction,Magnetic microspheres,DNA isolation,RNA,Food matrices,Polymerase chain reaction (PCR)

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