Short Report on DNA Marker at Candidate Locus

was performed with 2-5 p1 of DNA solution, 10 pmol of each primer: S-GATGCGCACAAGGTCCTGTC-3' and S-GCCAGCAGAGAGGTTTGCCT-3' (Jeunemaitre et al. 1992), 2 mM MgCl,, 20 mM (NH,),SO,, 75 mM Tris-HC1, pH 9.0, 0.01% Tween, 50 yM of each dNTP and 0.5 U Taq polymerase in a final volume of 25 yl. These pnmers were chosen to anneal just outside those used for the second round of PCR. After denaturation at 94°C for 3 min. DNA was amplified for 30 cycles of 94'C 1 min, 62°C 45 s, 72°C 30 s, followed by a final extension at 72'C for 5 min. Second-round PCR required 2 p1 of first-round product and 10 pmol of each primer: 5'-CAGGGT-