Dioscin Derived From Solanum Melongena L. "Usukawamarunasu" Attenuates Alpha-Msh-Induced Melanogenesis In B16 Murine Melanoma Cells Via Downregulation Of Phospho-Creb And Mitf
JOURNAL OF FOOD SCIENCE(2015)
摘要
This study aimed to chemically isolate and explore an antimelanogenesis inducer in extracts of Solanum melongena L. "Usukawamarunasu" eggplant. We successfully identified dioscin ([25R]-Spirost-5-en-3 beta-yl) 2-O-(6-deoxya-L-mannopyranosyl) -4-O-(6-deoxy-alpha-l-mannopyranosyl)-beta-D-glucopyranoside] in the plant, and examined the effects of alpha-melanocyte-stimulating hormone (MSH)-induced melanogenesis in B16 murine melanoma cells by this plant-derived dioscin. Immunoblot analysis suggested that dioscin reduced the expression of tyrosinase, tyrosinase-related protein-1 (TRP-1), and TRP-2, resulting in inhibition of intracellular production of melanin. In addition, dioscin caused reduction of phosphorylated cAMP-responsive element binding protein 1 transcription factors (CREB), which led to a reduction of microphthalmia-related transcription factor (MITF) in alpha-MSH-stimulated cells, but did not affect phosphorylation of extracellular signal-regulated kinase. Furthermore, dioscin significantly downregulated the expression of tyrosinase, TRP-1, and TRP-2, which led to the reduction of alpha-MSH-induced melanogenesis in B16 cells. These results suggest that dioscinmay decrease the level of MITF via inhibition of phosphorylation of CREB in alpha-MSH-induced melanogenesis in B16 cells.
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关键词
cAMP-responsive element binding protein 1 transcription factors, dioscin, melanogenesis, microphthalmia-related transcription factor, Solanum melongena L
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