208 Adiponectin Inhibits Endothelial Microparticle Generation: A Novel Mechanism Of Endothelial Protection

techniques: 1) single-layer running baseball stitch 2) doublelayer running baseball stitch 3) running horizontal mattress stitch combined with a baseball stitch. The aorta was pressurized with saline in a purpose-built apparatus which captured all leaked fluid for measurement of the leakage rate over time. The intra-aortic pressure was adjusted over 8 increments from 110 mmHg to supra-physiologic levels at 375 mmHg. Leakage rates were measured at each incremental pressure level and compared to previously measured baseline values. Eighteen trials were performed, with six trials in each of the three groups. RESULTS At all pressures, the horizontal mattress group was measured to have significantly less leakage when compared to single or double baseball stitch closure (p 0.05). The double layer baseball stitch shows a trend towards decreased leakage compared to the single layer closure. CONCLUSION This porcine model provides the first quantitative comparison of three commonly used aortotomy closure techniques. The running horizontal mattress stitch combined with a baseball stitch provides the greatest resistance to leakage throughout the normal, hypertensive, and supra-physiologic aortic pressures. This evidence suggests that this closure technique may provide superior hemostasis and resistance to rupture in the perioperative period.