The effects of metal ion PCR inhibitors on results obtained with the Quantifiler® Human DNA Quantification Kit

Forensic Science International: Genetics(2015)

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摘要
•DNA samples were treated with Al, Ca, Cu, Fe, Ni, and Pb.•Quantification was performed using the Quantifiler® Human DNA Quantification Kit.•The accuracy of human DNA quantification and inhibitor detection was assessed.•Al, Fe, and Pb-treatment yielded impaired human results before detection by IPC.•If possible inhibition, avoid drawing conclusions about sample from quantification.
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关键词
DNA quantification,PCR inhibition,Metal ions,6-Carboxyfluorescein,Fluorescence spectroscopy
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