Isolation and characterisation of Mauritius lowland day gecko Phelsuma guimbeaui microsatellite loci

Conservation Genetics Resources(2013)

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摘要
We isolated 315 sequences from a Phelsuma guimbeaui microsatellite-enriched genomic library. Primer sets were designed for 44 loci and used to genotype 29 unrelated individuals belonging to a population in the west of Mauritius. All the loci were polymorphic and the number of alleles ranged from 6 to 34. Mean observed and expected heterozygosity varied from 0.29 to 1.00 and 0.48 to 0.97, respectively. Thirteen loci displayed evidence of deviation from Hardy–Weinberg equilibrium, of which 11 also had an estimated null allele frequency ≥10 %. The microsatellite loci will be used to evaluate the population structure and genetic diversity of P . guimbeaui in Mauritius.
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关键词
Conservation,Gekkonidae,Microsatellite marker,Phelsuma,Reptile,Simple sequence repeats (SSRs)
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