An improved approach to align and embed multiple brain samples in a gelatin-based matrix for simultaneous histological processing.

⿢A method to simplify embedding of multiple brain samples was developed to facilitate histological processing.⿢Templates were designed to form brain-shaped cavities in a gelatin-based embedding matrix.⿢The cavities in the matrix allowed effortless positioning and alignment of brain samples during embedding.⿢The gelatin matrix effectively secured the samples during sectioning, staining and mounting.