Metabolic engineering of long chain-polyunsaturated fatty acid biosynthetic pathway in oleaginous fungus for dihomo-gamma linolenic acid production.

Chanikul Chutrakul,Sukanya Jeennor,Sarocha Panchanawaporn,Pattsarun Cheawchanlertfa,Sarinya Suttiwattanakul,Mayura Veerana,Kobkul Laoteng

Journal of Biotechnology（2016）

引用 30|浏览11

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摘要

•Production of dihomo-gamma linolenic acid was achieved by metabolic engineering.•Codon optimization of Δ6-elongase enhanced the rate of substrate conversion.•Fatty acid reconstitution did not affect growth and lipid phenotypes of A. oryzae.•Oleaginous fungus is a potential platform organism for production of lipid products.

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LC-PUFAs,DGLA,GLA,PGE1,PA,OA,LA,ARA,GAL1,PEG,ALA,STA,EPA,STC,TL,TLC,NL,FAMEs,ETA,GFP,toxA,nos3,DsRedExp,gpdA,TA,PE,PC,PL,DGAT

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