Antioxidant effects of vitamin C on hemoglobin-based oxygen carriers derived from human cord blood.

OBJECTIVE:To investigated the antioxidant protection effects of vitamin C (Vc) on the hemoglobin-based oxygen carriers (HBOCs) solution. METHODS:Three influential variables were studied including the molar ratio of Vc and hemoglobin, pH and temperature. The HBOCs molecules were characterized through spectral analysis, circular dichroism spectra, the oxygen binding curves and the molecular weights. RESULTS:The optimized conditions were molar ratios of above 3, pH of 7.5-8.0 and temperature of 4 °C. HBOCs characterization showed no differences before and after Vc addition. CONCLUSION:Antioxidant effects in HBOCs solution could be presented by Vc, indicating the potential application of Vc in HBOCs development.