Heterologous overexpression and biochemical characterization of the (galactophospho)lipase from Fusarium solani in Pichia pastoris that is expressed in planta

Raida Jallouli,Madiha Bou Ali,Mariam Charfeddine,Radhia Gargouri-Bouzid,Youssef Gargouri,Sofiane Bezzine

International Journal of Biological Macromolecules（2016）

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摘要

•A high level expression of a novel (phospho)lipase from F. solani was achieved.•The histidine extension of the recombinant enzyme does not affect its properties.•Possible involvement of this enzyme in the phytopathological process.

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关键词

F. solani (galactophospho)lipase,P. pastoris,Heterologous expression,Phytopathology

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