Combinatorial Biomolecular Nanopatterning for High-Throughput Screening of Stem-Cell Behavior.

Yacoub Y I Amin,Kasper Runager,Fabio Simoes,Adam Celiz,Vincenzo Taresco,Roberto Rossi,Jan J Enghild, Lisbeth A Abildtrup,David C E Kraft,Duncan S Sutherland,Morgan R Alexander,Morten Foss,Ryosuke Ogaki

ADVANCED MATERIALS（2016）

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摘要

A novel combinatorial biomolecular nanopatterning method is reported, in which multiple biomolecular ligands can be patterned in multiple nanoscale dimensions on a single surface. The applicability of the combinatorial platform toward cell-biology applications is demonstrated by screening the adhesion behavior of a population of human dental pulp stem cell (hDPSC) on 64 combinations of nanopatterned extracellular matrix (ECM) proteins in parallel.

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关键词

cell adhesion,combinatorial,high throughput,nanofabrication,protein patterning

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