Effects Of 530 Nm Monochromatic Light On Basic Fibroblast Growth Factor And Transforming Growth Factor-Beta 1 Expression In Muller Cells

AIM: To expose rat retinal Muller monochromatic light and investigate varying light illumination times on cells to 530 nm the influence of basic fibroblast growth factor (bFGF) and transforming growth factor-61 (TGF-beta 1) expression.METHODS: Three groups of rat retinal Willer cells cultured in vitro under a 530 nm monochromatic light were divided into 6, 12 and 24h experimental groups, while cells incubated under dark conditions served as the control group. The bFGF and TGF -beta 1 mRNA expression, protein levels and fluorescence intensity of the Mailer cells were analyzed.RESULTS: The bFGF mRNA expression and protein levels were significantly upregulated in Muller cells in all three experimental groups compared with the control group (P<0.05), while that of TGF-beta 1 was downregulated (P<0.05). Also, bFGF expression was positively correlated, but TGF -beta 1 expression was negatively correlated with illumination time. The largest changes for both cytokines were seen in the 24h group. The changes in bFGF and TGF -beta 1 fluorescence intensity were highest in the 24h group, and significant differences were observed among the experimental groups (P<0.05).CONCLUSION: The expressions of bFGF and TGF -beta 1 changed in a time -dependent manner in Muller cells exposed to 530 nm monochromatic light with 250 lx illumination intensity. Muller cells might play a role in the development of myopia by increasing bFGF expression or decreasing TGF -beta 1 expression. Changes in cytokine expression in retinal Muller cells may affect monochromatic light-induced myopia.