Comparison of inducible versus constitutive expression of plectasin on yields and antimicrobial activities in Pichia pastoris.

Xin Chen, Yong-hao Hu, Wei-di Chen,Wen-di Li, Zi-chao Huang, Yun Li,Yu-wen Luo,Yu-xia Huang,Yi-tai Chen,Kai Wang,Ling Li

Protein Expression and Purification(2016)

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摘要
•Differences were found in antibacterial activities and yields between inducible and constitutive expression of plectasin in Pichia pastoris.•The constitutive plectasin probably exhibits higher antibacterial activity.•The secretion process of constitutive plectasin is steadier and more controllable.•These findings may offer guidance for mass production and users of plectasin.
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关键词
Inducible expression,Constitutive expression,Plectasin,High level expression,Antimicrobial activity
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