Sensitive and specific detection of pine nut (Pinus spp.) by real-time PCR in complex food products

Cristiano Garino,Angelo De Paolis,Jean Daniel Coïsson,Daniela Manila Bianchi,Lucia Decastelli,Marco Arlorio

Food Chemistry（2016）

引用 23|浏览11

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摘要

•We set up a Taqman-based real time PCR method for the detection of Pinus spp.•The method displayed a very high efficiency and specificity for the genus Pinus.•Intrinsic LOD was 1pg of Pinus DNA, practical LOD was 0.1ppm of pine nuts powder.

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关键词

Pine nut,Pinus spp.,Food allergy,Real time PCR,Detection

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