Reply to |[ldquo]|Uncoupling diffusion and binding in FRAP experiments|[rdquo]|

Mario Brameshuber,Michaela Schwarzenbacher,Martin Kaltenbrunner,Clemens Hesch,Wolfgang Paster,Julian Weghuber,Bettina Heise,Alois Sonnleitner,Hannes Stockinger,Gerhard J Schütz

Nature Methods（2009）

引用 9|浏览13

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摘要

Brameshuber et al. reply: We thank Nevin Lambert for bringing this topic to discussion and pointing out the potential pitfalls in fluorescence recovery after photobleaching (FRAP) analysis1, which must also be considered in the case of our combined micropattern-FRAP method for the determination of interaction lifetimes2. As he pointed out, problems may arise when analyzing the binding of a mobile molecule to an immobile receptor3, 4, 5.

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