Long-span, mate-pair scaffolding and other methods for faster next-generation sequencing library creation

Cheng-Cang Wu,Rosa Ye,Svetlana Jasinovica,Megan Wagner,Ronald Godiska,Amy Hin-Yan Tong,Si Lok,Amanda Krerowicz, Curtis Knox,David Mead,Michael Lodes

Nature Methods（2012）

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摘要

The NxSeq ™ 40 kb Mate-Pair Cloning Kit facilitates the creation of scaffolds for de novo genome assembly. Supporting either Illumina or 454 sequencing, the kit produces long-span, mate-pair sequences with greater efficiency than existing protocols. In addition, NxSeq DNA Sample Prep Kits can be used to streamline workflow and speed up DNA library preparation for next-generation sequencing.

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关键词

Library construction,Next-generation sequencing,Life Sciences,general,Biological Techniques,Biological Microscopy,Biomedical Engineering/Biotechnology,Bioinformatics,Proteomics

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