In Vitro Selection for Small-Molecule-Triggered Strand Displacement and Riboswitch Activity.
ACS synthetic biology(2015)
摘要
An in vitro selection method for ligand-responsive RNA sensors was developed that exploited strand displacement reactions. The RNA library was based on the thiamine pyrophosphate (TPP) riboswitch, and RNA sequences capable of hybridizing to a target duplex DNA in a TPP regulated manner were identified. After three rounds of selection, RNA molecules that mediated a strand exchange reaction upon TPP binding were enriched. The enriched sequences also showed riboswitch activity. Our results demonstrated that small-molecule-responsive nucleic acid sensors can be selected to control the activity of target nucleic acid circuitry.
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关键词
SELEX,aptamer,in vitro selection,riboswitch,strand displacement
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