An insulated isothermal PCR method on a field-deployable device for rapid and sensitive detection of canine parvovirus type 2 at points of need.

•A CPV 2 iiPCR method for on-site detection of all circulating CPV 2 strains was developed.•Analytical sensitivity and specificity of CPV 2 iiPCR method in detecting CPV 2 DNA was examined.•Performance of iiPCR agreed with that of a reference qPCR in detecting CPV 2 in clinical samples.•The iiPCR method could provide rapid and accurate molecular detection of CPV at points of need.