An integrated view of redox and catalytic properties of B-type PpDyP from Pseudomonas putida MET94 and its distal variants.

Sónia Mendes,Vânia Brissos,Antonieta Gabriel,Teresa Catarino,David L Turner,Smilja Todorovic,Lígia O Martins

Archives of Biochemistry and Biophysics（2015）

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摘要

•The first report of redox potential of Cpd I/Fe3+ couple in a DyP peroxidase.•Distal Arg is essential for Cpd I formation and stabilization in the B-type PpDyP.•Mechanistic properties of DyPs are subfamily-dependent.

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关键词

Dye decolourising peroxidase,Pseudomonas putida,Site directed mutagenesis,Kinetics,Spectroscopy,Redox properties

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